Catalogue number: |
SMC-147A |
Reactivities: |
Human, Mouse |
Hosts: |
Mouse |
Applications: |
ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot |
Size: |
50ug |
Clone: |
2G5.G3 |
Accession: |
NP_001017963.2 |
Gene: |
3320 |
Swiss prot: |
P07900 |
Dilutions: |
1:2000 (WB) |
Form: |
Protein G Purified |
Storage buffer: |
PBS pH7.2, 50% glycerol |
Concentration: |
1mg/mL |
Isotype: |
IgG |
Shipping temp: |
Blue Ice or 4 |
Storage temp: |
-20 |
Research area: |
Heat Shock |
Certificate of analysis: |
0.5 ug/mL of SMC-147 was sufficient for detection of Hsp90alpha in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Scientific background: |
HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms (A) and (B), which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Des |
Alternative names: |
Hsp86, Hsp89A, Hsp90AA1, Hsp90Alpha, HspC1, HSPCA, HspCAL3 |
References: |
1. Nemoto, T. et al. (1997) J.Biol Chem. 272, 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266, 10099-10103.
3. Arlander SJH, et al. (2003) J Biol Chem 278, 52572-52577.
4. Pearl H, et al. (2001) Adv Protein Chem 59,157-186.
5. Neckers L, et al. |