Hsp90 total

Cat no: SMC-149B

Supplier: StressMarq Biosciences Inc.

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This antibody detects both (A) and (B) forms of Hsp90 equally well.

Catalogue number:	SMC-149B
Reactivities:	Human, Mouse
Hosts:	Mouse
Applications:	ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	200ug
Clone:	4F3.E8
Accession:	NP_001017963.2
Gene:	3320
Swiss prot:	P07900
Dilutions:	1:2000 (WB)
Form:	Protein G Purified
Storage buffer:	PBS pH7.2, 50% glycerol
Concentration:	1mg/mL
Isotype:	IgG1
Shipping temp:	Blue Ice or 4
Storage temp:	-20
Research area:	Heat Shock
Certificate of analysis:	0.5 ug/mL of SMC-149 was sufficient for detection of Hsp90alpha in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific background:	HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms (A) and (B), which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Des
Alternative names:	Hsp84, Hsp86, Hsp90A, Hsp90AA1, Hsp90AB1, Hsp90B, HspC1, HspC2, HspCAL1, HspCAL4
References:	1. Nemoto, T. et al. (1997) J.Biol Chem. 272, 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266, 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278, 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59,157-186. 5. Neckers L, et al.