Hsp90

Recognizes 90kDa proteins corresponding to the molecular mass of Hsp90. Hsp90(A) specific for human samples. Can isolate complexes of Hsp90, Src kinase and cec37.

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SPECIFICATIONS

Catalog Number

SMC-137C

Size

25ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Rabbit

Form

Protein G Purified

Clone

D7A

Gene Id

9031

Isotype

IgG

Accession

NP_001103255.1

Dilutions

1:500 (WB), 5ug with 20uL Protein A beads (IP )

References

1. Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296.\n2. Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880.\n3. Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19.\n4. Arlander SJH, et al. (2003) J

Swiss Prot

P11501

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS, 50% glycerol

Alternative Names

Hsp86, Hsp89A, Hsp90A, Hsp90AA1, HspC1, HspCA, HsoCAL3

Scientific Background

Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of

Certificate Of Analysis

2ug/ml was sufficient for detection of Hsp90(A) in 20ug of heat shocked HeLa cell lysate as well as in 100ng oh human Hsp90(A) protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.