Hsp90 complex

Immunoprecipitates 90kDa proteins corresponding to the molecular mass of Hsp90. Co-immunoprecipitates Hsp90 complexes, including Hsp70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2(A) (eIF-2(A)) kinase (HRI).

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SPECIFICATIONS

Catalog Number

SMC-109B

Size

200ug

Applications

IP

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

PEG Purified

Clone

8D3

Gene Id

3326

Isotype

IgM

Accession

NP_031381.2

References

1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186.\n3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.\n4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.\n5. Pratt W, Toft D. (1997) Endoc

Swiss Prot

P08238

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS, 50% glycerol

Scientific Background

Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a\nfunctional perspective, hsp90 participates in the folding,\nassembly, maturation, and stabilization of specific\nproteins as an integral component of

Certificate Of Analysis

Goat anti-mouse IgM was used to bind 25 ul of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 ul of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), b