Catalogue number: |
SMC-164C |
Reactivities: |
Human, Mouse, Rat, Chicken/Bird |
Hosts: |
Mouse |
Applications: |
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot |
Size: |
25ug |
Clone: |
3A3 |
Accession: |
NP_005336.3 |
Gene: |
3303 |
Swiss prot: |
P08107 |
Dilutions: |
ICC: 1/500, WB: 1/5000 by ECL; IP: 1-2ug |
Form: |
Protein G Purified |
Storage buffer: |
PBS pH7.2, 50% glycerol |
Concentration: |
1mg/mL |
Isotype: |
IgG1 |
Shipping temp: |
Blue Ice or 4 |
Storage temp: |
-20 |
Research area: |
Heat Shock |
Certificate of analysis: |
0.2 ug/mL of SMC-164 was sufficient for detection of Hsp70 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Scientific background: |
Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic r |
Alternative names: |
Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B |
References: |
1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17.
2. Rothman J. (1989) Cell 59: 591 -601.
3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
4. Bork P., Sander C. & V |