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Hsp70

Hsp70

Cat no: SMC-163D


Supplier: StressMarq Biosciences Inc.
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Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, and following heat shock, Hsp72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. IF staining of Hsp70 in heat shocked HeLa cells results in cytoplasmic staining.
Catalogue number: SMC-163D
Reactivities: Human, Mouse, Rat, Chicken/Bird
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Clone: 2A4
Accession: NP_005336.3
Gene: 3303
Swiss prot: P08107
Dilutions: ICC/IF: 1/100, IHC: 1/100, WB: 1/5000 (ECL)
Form: PEG purified
Storage buffer: PBS pH7.2, 50% glycerol
Concentration: 1mg/mL
Isotype: IgM
Shipping temp: Blue Ice or 4
Storage temp: -20
Research area: Heat Shock
Certificate of analysis: 0.2 ug/mL of SMC-163 was sufficient for detection of Hsp70 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgM:HRP as the secondary antibody.
Scientific background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic r
Alternative names: Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B
References: 1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 2. Rothman J. (1989) Cell 59: 591 -601. 3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 4. Bork P., Sander C. & V