Hsp22, 3C12-H11

Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

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SPECIFICATIONS

Catalog Number

SMC-187C

Size

25ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Protein G Purified

Clone

3C12-H11

Gene Id

26353

Isotype

IgG1 Kappa

Accession

NP_055180.1

Dilutions

1:2000 (WB)

References

1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6.\n2.Benndorf R., et al. (2001) J Biol Chem 276: 26753-\n26761.\n3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402.\n4.Kim M.V., et al. (2004) Biochem Biophys Res Commun\n325: 649-652.\n5. Wilhelmus M.M.,

Swiss Prot

Q9UJY1

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.4, 50% glycerol

Alternative Names

Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8, HspB8

Scientific Background

Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and\nthe human protein is most closely related to Hsp27.\nSimilar to most other sHSPs, Hsp22 is predominately\ntranscribed in skeletal muscle and heart, a

Certificate Of Analysis

1 ug/mL of SMC-187 was sufficient for detection of Hsp22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.