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Products HDEL
| Catalogue number: | SMC-175D |
| Hosts: | Mouse |
| Applications: | Western Blot |
| Size: | 100ug |
| Clone: | 2E7 |
| Accession: | N/A |
| Gene: | N/A |
| Swiss prot: | N/A |
| Dilutions: | 1:100-1:1000 (WB), 1:50-1:500 (IF) |
| Form: | Protein G Purified |
| Storage buffer: | PBS pH7.4, 50% glycerol |
| Concentration: | 1mg/mL |
| Isotype: | IgG2B |
| Shipping temp: | Blue Ice or 4 |
| Storage temp: | -20 |
| Research area: | Heat shock, trafficking |
| Certificate of analysis: | 1 ug/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Scientific background: | HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypept |
| References: | 1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271. |
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