Catalogue number: |
SMC-175C |
Hosts: |
Mouse |
Applications: |
Western Blot |
Size: |
25ug |
Clone: |
2E7 |
Accession: |
N/A |
Gene: |
N/A |
Swiss prot: |
N/A |
Dilutions: |
1:100-1:1000 (WB), 1:50-1:500 (IF) |
Form: |
Protein G Purified |
Storage buffer: |
PBS pH7.4, 50% glycerol |
Concentration: |
1mg/mL |
Isotype: |
IgG2B |
Shipping temp: |
Blue Ice or 4 |
Storage temp: |
-20 |
Research area: |
Heat shock, trafficking |
Certificate of analysis: |
1 ug/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Scientific background: |
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a
variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes;
participate in the translocation of polypept |
References: |
1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684.
2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697.
3. Entrez Gene: HDEL, Gene ID: 10945
4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271. |