FKBP52

Detects an ~52kDa protein representing FKBP52 in cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS PAGE.

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SPECIFICATIONS

Catalog Number

SMC-139C

Size

25ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ham

Form

Protein G Purified

Clone

Hi52C

Gene Id

2288

Isotype

IgG

Accession

NP_002005.1

Dilutions

1:2000 (WB), 5ug with 10-20uL Protein A beads (IP), 1:250 (IHC)

References

1. Cheung-Flynn J., Roberts P.J., Riggs D.L., and Smith D.F. (2003) J. Biol. Chem. 278(19): 17388-17394.\n2. Davies T.H., Ning Y.N., and Sanchez E.R. (2002)\nJ Biol. Chem. 277 (7): 4597-4600.\n3. Wu, B. et al. (2004) Proc. Natl. Acad. Sci. USA.\n101(22): 8348

Swiss Prot

Q02790

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS, 50% glycerol

Alternative Names

FK506 binding protein 4, FKBP4, FKBP59, HBI, Hsp56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein

Scientific Background

Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is co

Certificate Of Analysis

0.5ug/ml was sufficient for detection of FKBP52 in 20ug total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.