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SPECIFICATIONS
Catalog Number
SMC-182D
Size
100ug
Applications
WB
Hosts
Mouse
Reactivities
Hum
Form
Protein G purified
Clone
FIH 162c
Isotype
IgG1
Dilutions
10ug/mL( WB)
References
1. Stolze I.P., et al. (2004) J Bio Chem. 42719-42725. 2. Soilleux E.J., et al. (2005) Histopathology 47:602-\n610.\n3. Moon H., Han S., Park H., Choe J. (2010) Mol Cells.\n29(5): 471-474.\n4. Kroeze S.G., et al. (2010) Eur J Cancer. Epub.
Storage Temp
-20
Concentration
1mg/mL
Research Area
Oxidative Stress/ Cell Signaling
Shipping Temp
Blue Ice or 4
Storage Buffer
PBS pH7.4, 50% glycerol
Scientific Background
FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL\nrepresses HIF-1 transcriptional activity by disrupting the\ninteraction of HIF-1 with the transcriptional coactivators\nCBP/p300, and by recru
Certificate Of Analysis
1ug/mL of SMC-182 was sufficient for detection of FIH in 20ug of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Applications
ELISA, IP, WB
Hosts
Mouse
Reactivities
Hum
Applications
ELISA, ICC, IHC
Hosts
Mouse
Reactivities
Hum, Mouse, Rat, Bov
Applications
ELISA, ICC, IHC
Hosts
Mouse
Reactivities
Hum, Mouse, Rat, Bov
Applications
ELISA, ICC, IHC, WB
Hosts
Mouse
Reactivities
Hum
Applications
ELISA, ICC, IHC, WB
Hosts
Mouse
Reactivities
Hum
Applications
ELISA, ICC, IHC, WB
Hosts
Mouse
Reactivities
Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, Ham, Prc, Rabbit, Sheep
Applications
ELISA, ICC, IHC, WB
Hosts
Mouse
Reactivities
Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, Ham, Prc, Rabbit, Sheep
Hosts
Mouse
Reactivities
Hum, Mouse, Rat, Ch/Bird, GP, Prc
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