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Products DMPO, N1664A
| Catalogue number: | SMC-189D |
| Hosts: | Mouse |
| Applications: | ELISA, Immunoprecipitation, Western Blot |
| Size: | 100ug |
| Clone: | N1664A |
| Dilutions: | 1-10ug/mL (ELISA),10ug/mL (ICC), 25ug (IP) |
| Form: | Protein G Purified |
| Storage buffer: | TBS pH7.4, 0.05% sodium azide, 50% glycerol |
| Concentration: | 1mg/mL |
| Isotype: | IgG1 |
| Shipping temp: | Blue Ice or 4 |
| Storage temp: | -20 |
| Research area: | Oxidative Stress |
| Certificate of analysis: | A 1/1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Scientific background: | The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl- |
| Alternative names: | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide |
| References: | 1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473 |
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