CD74

Detects~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS PAGE immunoblots.

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SPECIFICATIONS

Catalog Number

SMC-116D

Size

100ug

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Mouse Immunoglobulin

Clone

PIN.1

Gene Id

972

Isotype

IgG

Accession

NP_001020329.1

Dilutions

1 ug/mL was sufficient for detection using colorimetric western blot analysis

References

1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9.\n2. Barrera CA., et al (2005) J Histochem Cytochem 53\n(12): 1481-9.\n3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816.\n4. Burton JD., et al.

Swiss Prot

P04233

Storage Temp

-20

Concentration

1mg/mL

Research Area

Cell Signaling

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.2, 50% glycerol

Alternative Names

DHLAG, HLA DR gamma, HLADG, p33, p35, Protein 41 antibody

Scientific Background

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to functio

Certificate Of Analysis

1ug/mL was sufficient for detection of SMC-116 in 20ug of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.