Alpha B Crystallin

Detects a band at ~20kDa (Predicted mol. weight is ~21kDa) corresponding to (A)B-crystallin. Does not cross-react with (A)A-crystallin, (B)L-crystallin, (B)H-crystallin, ?-crystallin, Hsp25, Hsp27 or Hsp47 proteins.

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SPECIFICATIONS

Catalog Number

SMC-159B

Size

200ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Bov

Form

Protein G Purified

Clone

1A7.D5

Gene Id

1410

Isotype

IgG1

Accession

NP_001876.1

Dilutions

1:2000 (WB)

References

1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA. 89(21):\n10449-10453.\n3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41:\n483-490\n4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.\n5. Bullard B. et al. (

Swiss Prot

P02511

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.2, in 50% glycerol

Alternative Names

AACRYA, CRYA2, CRYAB, CTPP2, HspB5, NY Ren 27 antigen antibody, alpha crystallin B antibody

Scientific Background

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian

Certificate Of Analysis

0.5 ug/mL of SMC-159 was sufficient for detection of 50ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.