Quick Search
Products Polyclonal Anti-Vinculin/VCL
| Catalogue number: | PA1781 |
| Price: | $200.00 |
| Reactivities: | Mouse |
| Applications: | Immunohistochemistry, Western Blot |
| Size: | 100ug/vial |
| Gene: | VCL |
| Swiss prot: | P18206 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al. (1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater-than-95% sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids. |
| References: | 1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, D. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell adhesion.Nature 430: 583-586, 2004. 2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992. 3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic cardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem. Biophys. Res. Commun. 349: 709-715, 2006. |
| Additional info: | A synthetic peptide corresponding to a sequence at the N-terminal of human VCL, identical to the related rat and mouse sequences. |
Get the Best Promotions
