Polyclonal Anti-TIMP1

Price

200.00 USD

Size

100ug/vial

Reactivities

Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Kenney, M. C., Chwa, M., Atilano, S. R., Tran, A., Carballo, M., Saghizadeh, M., Vasiliou, V., Adachi, W., Brown, D. J. Increased levels of catalase and cathepsin V/L2 but decreased TIMP-1 in keratoconus corneas: evidence that oxidative stress plays an role in this disorder. Invest. Ophthal. Vis. Sci. 46: 823-832, 2005. \n2. Willard, H. F., Durfy, S. J., Mahtani, M. M., Dorkins, H., Davies, K. E., Williams, B. R. G. Regional localization of the TIMP gene on the human X chromosome: extension of a conserved synteny and linkage group on proximal Xp. Hum. Genet. 81: 234-238, 1989.\n

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

TIMP1 (Tissue Inhibitor of Metalloproteinase 1), is a glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. Using a series of somatic cell hybrids segregating translocation and deletion X chromosomes, Willard et al. (1989) mapped the TIMP locus to Xp11.4-p11.23. By linkage analysis, TIMP was found to be located about 22 cM proximal to OTC. TIMP is identical to the collagenase inhibitor. Human collagenase inhibitor (HCI) appears to play a major role in modulating the activity of interstitial collagenase as well as a number of connective tissue metalloendoproteases. Kenney et al. (2005) found that keratoconus corneas exhibited a 1.8-fold decrease in TIMP1 mRNA and a 2.8-fold decrease in protein compared with normal (physiologic) corneas.