Polyclonal Anti-SNRPN

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Bielinska, B., Blaydes, S. M., Buiting, K., Yang, T., Krajewska-Walasek, M., Horsthemke, B., \nBrannan, C. I. De novo deletions of SNRPN exon 1 in early human and mouse embryos result in a \npaternal to maternal imprint switch. Nature Genet. 25: 74-78, 2000.\n2. Cattanach, B. M., Barr, J. A., Evans, E. P., Burtenshaw, M., Beechey, C. V., Leff, S. E., Brannan, C. \nI., Copeland, N. G., Jenkins, N. A., Jones, J. A candidate mouse model for Prader-Willi syndrome \nwhich shows an absence of Snrpn expression. Nature Genet. 2: 270-274, 1992.\n3. Geuns, E., De Rycke, M., Van Steirteghem, A., Liebaers, I. Methylation imprints of the imprint \ncontrol region of the SNRPN-gene in human gametes and preimplantation embryos. Hum. Molec. \nGenet. 12: 2873-2879, 2003.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

SNRPN(Small Nuclear Ribonucleoprotein Polypeptide N), also called SMN, is a bicistronic imprinted \ngene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and \nthe SNRPN upstream reading frame (SNURF) polypeptide. The protein encoded by this gene is one \npolypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family.\nSNRPN also encodes a long alternatively spliced transcript containing several small nucleolar RNAs \n(snoRNAs) and extends downstream to partially overlap the UBE3A gene in the antisense orientation. \nPWS arises from loss of function of genes in this region expressed exclusively from the paternal \nchromosome, suggesting that SNRPN may play a role in its etiology. The SNRPN gene is mapped \non 15q11.2. Analysis of maternal DNA and of SNRPN cDNA confirmed that the maternal allele is not \nexpressed in fetal brain and heart. Deletions in the transcription unit of the imprinted SNRPN gene \noccur in patients who have PWS or Angelman syndrome because of a parental imprint switch failure in \nthis chromosomal domain.