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Polyclonal Anti-SMAD2/3

Polyclonal Anti-SMAD2/3

Cat no: PA1073

Supplier: Boster Immunoleader
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Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2/3 (SMAD2/3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Catalogue number: PA1073
Price: $200.00
Reactivities: Human, Mouse, Rat
Applications: Immunohistochemistry, Western Blot
Size: 100ug/vial
Gene: SMAD2/3
Swiss prot: Q15796
Form: Lyophilized
Format: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Storage temp: "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. "
Scientific background: SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Transforming growth factor (TGF)-beta stimulation leads to phosphorylation and activation of Smad2 and Smad3, which form complexes with Smad4 that accumulate in the nucleus and regulate transcription of target genes. Smad2 and Smad3 share highly homology. SMAD2/SMAD3 signal transduction appeared to be important in the regulation of muscle-specific genes. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development.Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development.
References: 1. Riggins G.J., Thiagalingam S., Rosenblum E., Weinstein C.L., Kern S.E., Hamilton S.R., Willson J.K.V., Markowitz S.D., Kinzler K.W., Vogelstein B.V.;"Mad-related genes in the human.";Nat. Genet. 13:347-349(1996). 2. Zhang Y., Feng X.-H., Wu R.-Y., Derynck R.;"Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.";Nature 383:168-172(1996). 3. Inman, G. J.; Nicolas, F. J.; Hill, C. S. : Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.
Additional info: A synthetic peptide corresponding to a sequence at the N-terminal of human SMAD2/3(32-47aa EQNGQEEKWCEKAVKS), identical to the related mouse and rat sequences.
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