Polyclonal Anti-RAB5A

Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A (RAB5A) detection. Tested with WB, IHC-P in Human;Mouse;Rat. \n\n

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1773

Size

100ug/vial

Applications

IHC, WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

RAB5A

References

1. Bucci, C., Parton, R. G., Mather, I. H., Stunnenberg, H., Simons, K., Hoflack, B., Zerial, M. The small \nGTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell 70: 715-728, \n1992.\n2. Kitano, M., Nakaya, M., Nakamura, T., Nagata, S., Matsuda, M. Imaging of Rab5 activity identifies \nessential regulators for phagosome maturation. Nature 453: 241-245, 2008.\n3. Lanzetti, L., Rybin, V., Malabarba, M. G., Christoforidis, S., Scita, G., Zerial, M., Di Fiore, P. P. The \nEps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5. Nature \n408: 374-377, 2000.

Swiss Prot

P20339

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence in the middle region\nof human RAB5A, identical to the related rat and mouse sequences .

Scientific Background

RAB5A(Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by \nthe RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of \nmembrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. Tuberin \nfunctions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis. Ectopically \nexpressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal \ncompartments and stimulated the enlargement of endosomes in cultured cortical neurons. RAB5 is \nindispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular \nruffling. RAB5 signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating \nprotein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin \ncoat encapsulating phagosomes. RAB5 activation was either continuous or repetitive for up to 10 \nminutes, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative \nmutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome \nmaturation.