Polyclonal Anti-PROM1

Rabbit IgG polyclonal antibody for Prominin-1 (PROM1) detection. Tested with WB, IHC-P in Human;Rat.

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA2049

Size

100ug/vial

Applications

IHC, WB

Reactivities

Hum, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

PROM1

References

1. Maw, M. A., Corbeil, D., Koch, J., Hellwig, A., Wilson-Wheeler, J. C., Bridges, R. J., Kumaramanickavel, G., John, S., Nancarrow, D., Roper, K., Weigmann, A., Huttner, W. B., Denton, M. J. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Molec. Genet. 9: 27-34, 2000. \n2. Singh, S. K., Clarke, I. D., Terasaki, M., Bonn, V. E., Hawkins, C., Squire, J., Dirks, P. B. Identification of a cancer stem cell in human brain tumors. Cancer Res. 63: 5821-5828, 2003.\n3. Torrente, Y., Belicchi, M., Sampaolesi, M., Pisati, F., Meregalli, M., D'Antona, G., Tonlorenzi, R., Porretti, L., Gavina, M., Mamchaoui, K., Pellegrino, M. A., Furling, D., Mouly, V., Butler-Browne, G. S., Bottinelli, R., Cossu, G., Bresolin, N. Human circulating AC133(+) stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle. J. Clin. Invest. 114: 182-195, 2004.\n

Swiss Prot

O43490

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence at the C-terminal of human PROM1, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Scientific Background

PROM1 (Prominin 1), also called CD133, is a glycoprotein. Maw et al. (2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al. (2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al. (2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor.