Polyclonal Anti-PRDX1

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Neumann, C. A., Krause, D. S., Carman, C. V., Das, S., Dubey, D. P., Abraham, J. L., Bronson, R. T., \nFujiwara, Y., Orkin, S. H., Van Etten, R. A.Essential role for the peroxiredoxin Prdx1 in erythrocyte \nantioxidant defence and tumour suppression. Nature 424: 561-565, 2003.\n2. Prosperi, M.-T., Apiou, F., Dutrillaux, B., Goubin, G. Organization and chromosomal assignment of \ntwo human PAG gene loci: PAGA encoding a functional gene and PAGB a processed \npseudogene. Genomics 19: 236-241, 1994.\n3. Yan, Y., Sabharwal, P., Rao, M., Sockanathan, S. The antioxidant enzyme Prdx1 controls neuronal \ndifferentiation by thiol-redox-dependent activation of GDE2. Cell 138: 1209-1221, 2009.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical \nroles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and \nact as a molecular chaperone, respectively. This gene encodes a member of the peroxiredoxin family of \nantioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The PRDX1 gene is \nmapped on 1p34.1. Prdx1 was expressed in differentiating motor neuron cells in developing embryonic \nchicken and mouse spinal cords. mmunoprecipitation analysis showed that GDE2 interacted directly \nwith PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells. This protein \nmay have a proliferative effect and play a role in cancer development or progression. In differentiating \nspinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between \nthe Gde2 N- and C-terminal domains. An intramolecular disulfide bond between the GDE2 N- and \nC-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 \nfor the induction of motor neuron differentiation.