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Products Polyclonal Anti-POU2AF1
| Catalogue number: | PA1506 |
| Price: | $200.00 |
| Reactivities: | Mouse, Rat |
| Applications: | Immunohistochemistry, Western Blot |
| Size: | 100ug/vial |
| Gene: | POU2AF1 |
| Swiss prot: | Q64693 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice. |
| References: | 1. Casellas, R., Jankovic, M., Meyer, G., Gazumyan, A., Luo, Y., Roeder, R. G., Nussenzweig, M. C.OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes.Cell 110: 575-585, 2002. 2. Siegel, R., Kim, U., Patke, A., Yu, X., Ren, X., Tarakhovsky, A., Roeder, R. G.Nontranscriptional regulation of SYK by the coactivator OCA-B is required at multiple stages of B cell development.Cell 125: 761-774, 2006. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of mouse POU2AF1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids. |
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