Quick Search
Products Polyclonal Anti-P27
| Catalogue number: | PA1063 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Immunohistochemistry, Western Blot |
| Size: | 100ug/vial |
| Gene: | CDKN1B |
| Swiss prot: | P46527 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas. |
| References: | 1. Martin E, Cacheux V, Cave H, Lapierre JM, Le Paslier D, Grandchamp B.Localization of the CDKN4/p27Kip1 gene to human chromosome 12p12.3. Hum Genet. 1995 Dec; 96(6):668-70. 2. Sheaff RJ, Groudine M, Gordon M, Roberts JM, Clurman BE.Cyclin E-CDK2 is a regulator of p27Kip1. Genes Dev. 1997 Jun 1; 11(11):1464-78. 3. Braun-Dullaeus RC, Mann MJ, Ziegler A, von der Leyen HE, Dzau VJ.A novel role for the cyclin-dependent kinase inhibitor p27(Kip1) in angiotensin II-stimulated vascular smooth muscle cell hypertrophy. J Clin Invest. 1999 Sep; 104(6):815-23. 4. Carrano AC, Eytan E, Hershko A, Pagano M.SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat Cell Biol. 1999 Aug; 1(4):193-9. 5. Gopfert U, Kullmann M, Hengst L.Cell cycle-dependent translation of p27 involves a responsive element in its 5'-UTR that overlaps with a uORF. Hum Mol Genet. 2003 Jul 15; 12(14):1767-79. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human P27, different from the related rat and mouse sequences by one amino acid. |
Get the Best Promotions
