Polyclonal Anti-NRP2

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Chen, H., Bagri, A., Zupicich, J. A., Zou, Y., Stoeckli, E., Pleasure, S. J., Lowenstein, D. H., Skarnes, \nW. C., Chedotal, A., Tessier-Lavigne, M.Neuropilin-2 regulates the development of select cranial \nand sensory nerves and hippocampal mossy fiber projections. Neuron 25: 43-56, 2000.\n2. Gong, S., Zheng, C., Doughty, M. L., Losos, K., Didkovsky, N., Schambra, U. B., Nowak, N. J., \nJoyner, A., Leblanc, G., Hatten, M. E., Heintz, N.A gene expression atlas of the central nervous \nsystem based on bacterial artificial chromosomes. Nature 425: 917-925, 2003.\n3. Rossignol, M., Beggs, A. H., Pierce, E. A., Klagsbrun, M. Human neuropilin-1 and neuropilin-2 map \nto 10p12 and 2q34, respectively.Genomics 57: 459-460, 1999.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of \nreceptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in \nChinese hamster ovary cells. The NRP2 gene is mapped on 2q33.3. The NRP2 gene contains 17 \nexons and spans about 112 kb. Sema3b can act to repulse axons expressing neuropilin-2 and can \nantagonize the repulsive action of Sema3a on axonal growth cones expressing neuropilin-1. This \nprotein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with \nnull mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 \n(PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine\nnumber and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and \nexcitatory synapses in dissociated neurons in vitro, and in Npn2-null brain slices cortical layer V and \ndentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These \ndisparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Npn2 to \napical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.