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Products Polyclonal Anti-NR3C2
| Catalogue number: | PA1594 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | NR3C2 |
| Swiss prot: | P08235 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | NR3C2 (nuclear receptor subfamily 3, group C, member 2) also known as MR (mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. NR3C2 is a receptor with high affinity for mineralocorticoids. It belongs to the nuclear receptor family where the ligand diffuses into cells, interacts with the receptor and results in a signal transduction affecting specific gene expression in the nucleus. MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system (hippocampus), brown adipose tissue and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports (mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body. The receptor is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol. In intact animals, the mineralocorticoid receptor is "protected" from glucocorticoids by co-localization of an enzyme, 11 |
| References: | 1.Alnemri, E. S., Maksymowych, A. B., Robertson, N. M., Litwack, G.Overexpression and characterization of the human mineralocorticoid receptor.J. Biol. Chem. 266: 18072-18081, 1991. 2.Hellal-Levy, C., Fagart, J., Souque, A., Wurtz, J.-M., Moras, D., Rafestin-Oblin, M.-E.Crucial role of the H11-H12 loop in stabilizing the active conformation of the human mineralocorticoid receptor.Molec. Endocr. 14: 1210-1221, 2000. 3.Le Menuet, D., Isnard, R., Bichara, M., Viengchareun, S., Muffat-Joly, M., Walker, F., Zennaro, M.-C., Lombes, M.Alteration of cardiac and renal functions in transgenic mice overexpressing human mineralocorticoid receptor.J. Biol. Chem. 276: 38911-38920, 2001. |
| Additional info: | A synthetic peptide mapping at the C-terminal of human NR3C2, identical to the related mouse and rat sequences. |
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