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Polyclonal Anti-NOXA1

Polyclonal Anti-NOXA1

Cat no: PA1930

Supplier: Boster Immunoleader
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Rabbit IgG polyclonal antibody for NADPH oxidase activator 1 (NOXA1) detection. Tested with WB in Human.
Catalogue number: PA1930
Price: $200.00
Reactivities: Human
Applications: Western Blot
Size: 100ug/vial
Gene: NOXA1
Swiss prot: Q86UR1
Form: Lyophilized
Format: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Storage temp: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.
Scientific background: NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
References: 1. Banfi, B., Clark, R. A., Steger, K., Krause, K.-H. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J. Biol. Chem. 278: 3510-3513, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/25/2007. 3. Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., Sumimoto, H. Novel human homologues of p47(phox) and p67(phox) participate in activation of superoxide-producing NADPH oxidases. J. Biol. Chem. 278: 25234-25246, 2003.
Additional info: A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.
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