Polyclonal Anti-NOX4

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Ago, T., Kitazono, T., Ooboshi, H., Iyama, T., Han, Y. H., Takada, J., Wakisaka, M., Ibayashi, S., Utsumi, H., Iida, M. Nox4 as the major catalytic component of an endothelial NAD(P)H oxidase. Circulation 109: 227-233, 2004.\n2. Geiszt, M., Kopp, J. B., Varnai, P., Leto, T. L. Identification of Renox, an NAD(P)H oxidase in kidney. Proc. Nat. Acad. Sci. 97: 8010-8014, 2000.\n3. Shiose, A., Kuroda, J., Tsuruya, K., Hirai, M., Hirakata, H., Naito, S., Hattori, M., Sakaki Y., Sumimoto, H. A novel superoxide-producing NAD(P)H oxidase in kidney. J. Biol. Chem. 276: 1417-1423, 2001.\n

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

NOX4(NADPH oxidase 4), also called RENAL NADPH OXIDASE or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al. (2000) stated that the nucleotide sequence of RENOX matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to RENOX. By FISH, Shiose et al. (2001) mapped the NOX4 gene to chromosome 11q14.2-q21. Geiszt et al. (2000) found that NIH 3T3 fibroblasts overexpressing transfected RENOX showed increased production of superoxide and developed signs of cellular senescence. They suggested that RENOX, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al. (2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.