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Products Polyclonal Anti-MLL
| Catalogue number: | PA1770 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | MLL |
| Swiss prot: | Q03164 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | MLL(Myeloid/lymphoid or mixed Lineage Leukemia gene),also called MLL1, ALL1 or CXX7, is a frequent target for recurrent translocations in acute leukemias that may be characterized as acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), or mixed lineage (biphenotypic) leukemia (MLL). The MLL gene encodes a DNA-binding protein that methylates histone H3 lys4 (H3K4) and positively regulates expression of target genes, including multiple HOX genes. The MLL gene is mapped on 11q23.3. A leukemogenicMLL fusion protein that activates HOX expression had no effect on histone methylation, suggesting a distinct mechanism for gene regulation by MLLand MLL fusion proteins. The MLL gene encodes a large nuclear protein that is required for the maintenance of HOX gene expression. MLL is cleaved at 2 conserved sites to generate an N-terminal 320-kD fragment (N320) and a C-terminal 180-kD fragment (C180), which heterodimerize to stabilize the complex and confer its subnuclear destination. RNA interference-mediated knockdown of taspase-1 in HeLa cells resulted in the appearance of unprocessed MLL and the loss of proper HOX gene expression. |
| References: | 1. Armstrong, S. A., Staunton, J. E., Silverman, L. B., Pieters, R., den Boer, M. L., Minden, M. D., Sallan, S. E., Lander, E. S., Golub, T. R., Korsmeyer, S. J. MLL translocations specify a distinct gene expression profile that distinguishes a unique leukemia. Nature Genet. 30: 41-47, 2002. 2. Bruch, J., Wilda, M., Teigler-Schlegel, A., Harbott, J., Borkhardt, A., Metzler, M. Occurrence of an MLL/LAF4 fusion gene caused by the insertion ins(11;2)(q23;q11.2q11.2) in an infant with acute lymphoblastic leukemia. (Letter) Genes Chromosomes Cancer 37: 106-109, 2003. 3. Caligiuri, M. A., Strout, M. P., Oberkircher, A. R., Yu, F., de la Chapelle, A., Bloomfield, C. D. The partial tandem duplication of ALL1 in acute myeloid leukemia with normal cytogenetics or trisomy 11 is restricted to one chromosome. Proc. Nat. Acad. Sci. 94: 3899-3902, 1997. |
| Additional info: | A synthetic peptide corresponding to a sequence at the N-terminal of human MLL, identical to the related mouse sequence. |
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