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Products Polyclonal Anti-MGMT
| Catalogue number: | PA1408 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | MGMT |
| Swiss prot: | P16455 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | Methylated-DNA-protein-cysteine methytransferase (MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al. (1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al. (1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis. |
| References: | 1. Tano K, Shiota S, Collier J, Foote RS, Mitra S (January 1990). "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine". Proc. Natl. Acad. Sci. U.S.A. 87 (2): 686 |
| Additional info: | A synthetic peptide corresponding to a sequence in the middle region of human MGMT, different from the related rat and mouse sequences by one amino acid. |
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