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Products Polyclonal Anti-LPL
| Catalogue number: | PA1304 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat, Bovine |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | LPL |
| Swiss prot: | P06858 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. Lipoprotein lipase has been difficult to purify, and its protein sequence remained undetermined until it could be deduced from the nucleotide sequence of its cDNA. The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. Analysis of the sequence indicated that human lipoprotein lipase, hepatic lipase, and pancreatic lipase are members of a gene family. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. |
| References: | 1. Wion, K. L.; Kirchgessner, T. G.; Lusis, A. J.; Schotz, M. C.; Lawn, R. M. : Human lipoprotein lipase complementary DNA sequence. Science 235: 1638-1641, 1987. 2. Gotoda T., Senda M., Gamou T., Furuichi Y., Oka K.;Nucleotide sequence of human cDNA coding for a lipoprotein lipase (LPL) cloned from placental cDNA library."; Nucleic Acids Res. 17:2351-2352(1989). 3. Lo, J. Y.; Smith, L. C.; Chan, L. : Lipoprotein lipase: role of intramolecular disulfide bonds in enzyme catalysis. Biochem. Biophys. Res. Commun. 206: 266-271, 1995. 4. Lopez-Miranda, J.; Cruz, G.; Gomez, P.; Marin, C.; Paz, E.; Perez-Martinez, P.; Fuentes, F. J.; Ordovas, J. M.; Perez-Jimenez, F. : The influence of lipoprotein lipase gene variation on postprandial lipoprotein metabolism. J. Clin. Endocr. Metab. 89: 4721-4728, 2004. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human LPL, different from the related mouse and rat sequences by two amino acids. |
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