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Products Polyclonal Anti-LCAT
| Catalogue number: | PA1967 |
| Price: | $200.00 |
| Reactivities: | Human, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | LCAT |
| Swiss prot: | P04180 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed 'reverse cholesterol transport.' The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000). |
| References: | 1. Azoulay, M., Henry, I., Tata, F., Weil, D., Grzeschik, K. H., Chaves, E., McIntyre, N., Williamson, R., Humphries, S. E., Junien, C. The structural gene for lecithin:cholesterol acyl transferase (LCAT) maps to 16q22. Ann. Hum. Genet. 51: 129-136, 1987. 2. Frohlich, J., McLeod, R., Hon, K. Lecithin:cholesterol acyl transferase (LCAT). Clin. Biochem. 15: 269-278, 1982. 3. Jonas, A. Lecithin cholesterol acyltransferase. Biochim. Biophys. Acta 1529: 245-256, 2000. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human LCAT, different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids. |
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