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Products Polyclonal Anti-K-ras
| Catalogue number: | PA1457 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | KRAS |
| Swiss prot: | P01116 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | K-ras, V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, is a protein that in humans is encoded by the KRAS gene. Like other members of the Ras family, the K-ras protein is a GTPase and is an early player in many signal transduction pathways. K-ras is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. The K-ras gene spans 38 kb and contains 4 exons. By in situ hybridization, the K-ras gene is mapped to chromosome 12p12.1-p11.1. K-ras acts as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. K-ras binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP |
| References: | 1. Popescu, N. C., Amsbaugh, S. C., DiPaolo, J. A., Tronick, S. R., Aaronson, S. A., Swan, D. C.Chromosomal localization of three human ras genes by in situ molecular hybridization.Somat. Cell Molec. Genet. 11: 149-155, 1985. 2. Porta, M., Malats, N., Jariod, M., Grimalt, J. O., Rifa, J., Carrato, A., Guarner, L., Salas, A., Santiago-Silva, M., Corominas, J. M., Andreu, M., Real, F. X.Serum concentrations of organochlorine compounds and K-ras mutations in exocrine pancreatic cancer. 3. Weinberg, R. A.Fewer and fewer oncogenes.Cell 30: 3-4, 1982. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human K-ras, identical to the related mouse and rat sequence. |
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