Quick Search
Products Polyclonal Anti-HSP27
| Catalogue number: | PA1121 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Immunohistochemistry, Western Blot |
| Size: | 100ug/vial |
| Gene: | HSPB1 |
| Swiss prot: | P04792 |
| Form: | Liquid |
| Format: | Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. The deduced 199-amino acid HSP27 protein shows sequence similarity to mammalian alpha-crystallins. Approximately 20% of its residues are susceptible to phosphorylation. The HSP27 gene, which is mapped to 7q11.23 and has 3 exons, produced a 2.2-kb transcript in an in vitro transcription assay. Decreasing ROS in cells expressing mutant huntingtin, HSP27 protects cells against oxidative stress. In other words, HSP27 is a suppressor of polyglutamine (polyQ)-mediated cell death. Furthermore, MAPKAPK5 is a major stress-activated kinase that can phosphorylate HSP27 in vitro. |
| References: | 1. Hickey, E.; Brandon, S. E.; Sadis, S.; Smale, G.; Weber, L. A. : Molecular cloning of sequences encoding the human heat-shock proteins and their expression during hyperthermia. Gene 43: 147-154, 1986. 2. Wyttenbach, A.; Sauvageot, O.; Carmichael, J.; Diaz-Latoud, C.; Arrigo, A.-P.; Rubinsztein, D. C. : Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11: 1137-1151, 2002. 3. New, L.; Jiang, Y.; Zhao, M.; Liu, K.; Zhu, W.; Flood, L. J.; Kato, Y.; Parry, G. C. N.; Han, J. : PRAK, a novel protein kinase regulated by the p38 MAP kinase. EMBO J. 17: 3372-3384, 1998. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human HSP27, different from the related mouse sequence by two amino acids. |
Get the Best Promotions
