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Polyclonal Anti-GAD67

Polyclonal Anti-GAD67

Cat no: PA1036

Supplier: Boster Immunoleader
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Rabbit IgG polyclonal antibody for Glutamate decarboxylase 1 (GAD1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Catalogue number: PA1036
Price: $200.00
Reactivities: Human, Mouse, Rat
Applications: Immunohistochemistry, Western Blot
Size: 100ug/vial
Gene: GAD1
Swiss prot: Q99259
Form: Liquid
Format: Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.
Storage temp: "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. "
Scientific background: Glutamic acid decarboxylase (GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 585 amino acid residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594 amino acid residues. GAD67 gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD67 gene contains an additional exon (exon 0) that together with part of exon 1, specifies the 5' untranslated region of GAD67 mRNA. Human GAD67 shows 65% identity to GAD65 and is located in 2q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.
References: 1. Kelly, C. D.; Edwards, Y.; Johnstone, A. P.; Harfst, E.; Nogradi, A.; Nussey, S. S.; Povey, S.; Carter, N. D. : Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase. Ann. Hum. Genet. 56: 255-265, 1992. 2. Bu, D.-F.; Erlander, M. G.; Hitz, B. C.; Tillakaratne, N. J. K.; Kaufman, D. L.; Wagner-McPherson, C. B.; Evans, G. A.; Tobin, A. J. : Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992. 3. Bu, D.-F.; Tobin, A. J. : The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD-67 and GAD-65) suggests that they derive from a common ancestral GAD. Genomics 21: 222-228, 1994.
Additional info: A synthetic peptide corresponding to a sequence at the N-terminal of human GAD67, identical to the related rat and mouse sequences.
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