Polyclonal Anti-ECI1

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Janssen, U., Fink, T., Lichter, P., Stoffel, W.Human mitochondrial 3,2-trans-enoyl-CoA isomerase \n(DCI): gene structure and localization to chromosome 16p13.3.Genomics 23: 223-228, 1994.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

ECI1/DCI(Dodecenoyl-CoA Delta Isomerase), also known as 3,2-trans-enoyl-CoA isomerase, is an \nenzyme that catalyzes the conversion of cis-or trans-double bonds of fatty acids at gamma-carbon\n(position 3) to trans double bonds at beta-carbon (position 2). It plays a particularly important role in the \nmetabolism of unsaturated fatty acids. All classes of enoyl-CoA isomerases belong to a family of \nenzymes, the hydratase/isomerase or crotonase superfamily, and when examined with x-ray \ncrystallography, exhibit a common structural feature of the family, the N-terminal core with a spiral fold \ncomposed of four turns, each turn consisting of two beta-sheets and one alpha-helix. Dodecenoyl-CoA \nDelta Isomerase is involved in the beta-oxidation, one of the most frequently used pathways in fatty acid \ndegradation, of unsaturated fatty acids with double bonds at odd-numbered carbon positions. It does so \nby shifting the position of the double bonds in the acyl-CoA intermediates and converting 3-cis or \ntrans-enoyl-CoA to 2-trans-enoyl-CoA.