Polyclonal Anti-CYP2E1

Rabbit IgG polyclonal antibody for Cytochrome P450 2E1 (CYP2E1) detection. Tested with WB in Human.

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1908

Size

100ug/vial

Applications

WB

Reactivities

Hum

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

CYP2E1

References

1. Nebert, D. W.; Adesnik, M.; Coon, M. J.; Estabrook, R. W.; Gonzalez, F. J.; Guengerich, F. P.; Gunsalus, I. C.; Johnson, E. F.; Kemper, B.; Levin, W.; Phillips, I. R.; Sato, R.; Waterman, M. R. : The P450 gene superfamily: recommended nomenclature. DNA 6: 1-11, 1987.\n2. Song, B.-J.; Gelboin, H. V.; Park, S.-S.; Yang, C. S.; Gonzalez, F. J. : Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. J. Biol. Chem. 261: 16689-16697, 1987.\n3. Hayashi, S.; Watanabe, J.; Kawajiri, K. : Genetic polymorphisms in the 5-prime-flanking region change transcriptional regulation of the human cytochrome P450IIE1 gene. J. Biochem. 110: 559-565, 1991.\n

Swiss Prot

P05181

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence at the N-terminal of human CYP2E1.

Scientific Background

CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans?and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.