Polyclonal Anti-CUL2

Rabbit IgG polyclonal antibody for Cullin-2 (CUL2) detection. Tested with WB in Human;Mouse;Rat.

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SPECIFICATIONS

Price

200.00 USD

Catalog Number

PA1877

Size

100ug/vial

Applications

WB

Reactivities

Hum, Mouse, Rat

Form

Lyophilized

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Gene Id

CUL2

References

1. Clifford, S. C., Walsh, S., Hewson, K., Green, E. K., Brinke, A., Green, P. M., Gianelli, F., Eng, C., Maher, E. R. Genomic organization and chromosomal localization of the human CUL2 gene and the role of von Hippel-Lindau tumor suppressor-binding protein (CUL2 and VBP1) mutation and loss in renal-cell carcinoma development. Genes Chromosomes Cancer 26: 20-28, 1999. \n2. Lonergan, K. M., Iliopoulos, O., Ohh, M., Kamura, T., Conaway, R. C., Conaway, J. W., Kaelin, W. G., Jr.Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor suppressor protein requires binding to complexes containing elongins B/C and Cul2. Molec. Cell. Biol. 18: 732-741, 1998.\n3. Pause, A., Lee, S., Worrell, R. A., Chen, D. Y. T., Burgess, W. H., Linehan, W. M., Klausner, R. D. The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Nat. Acad. Sci. 94: 2156-2161, 1997\n

Swiss Prot

Q13617

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Additional Info

A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2, different from the related mouse and rat sequences by one amino acid.

Scientific Background

Cullin 2(CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al. (1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al. (1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1 (603134). Lonergan et al. (1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.