Polyclonal Anti-CISH

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum, Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Hu, G., Zhou, R., Liu, J., Gong, A.-Y., Eischeid, A. N., Dittman, J. W., Chen, X.-M. MicroRNA-98 and \nlet-7 confer cholangiocyte expression of cytokine-inducible Src homology 2-containing protein in \nresponse to microbial challenge. J. Immun. 183: 1617-1624, 2009.\n2. Khor, C. C., Vannberg, F. O., Chapman, S. J., Guo, H., Wong, S. H., Walley, A. J., Vukcevic, D., \nRautanen, A., Mills, T. C., Chang, K.-C., Kam, K.-M., Crampin, A. C., and 23 others. CISH and \nsusceptibility to infectious diseases. New Eng. J. Med. 362: 2092-2101, 2010.\n3. Uchida, K., Yoshimura, A., Inazawa, J., Yanagisawa, K., Osada, H., Masuda, A., Saito, T., Takahashi, \nT., Miyajima, A., Takahashi, T. Molecular cloning of CISH, chromosome assignment to 3p21.3, and \nanalysis of expression in fetal and adult tissues. Cytogenet. Cell Genet. 78: 209-212, 1997

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important \nnegative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling \n(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine \nsignaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are \nassociated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is \nmapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on \nchromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by \nlipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved \ndownregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression \nof CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and \nenhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.