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Products Polyclonal Anti-CHEK2
| Catalogue number: | PA1655 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | CHEK2 |
| Swiss prot: | O96017 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing. |
| Scientific background: | CHEK2(checkpoint kinase 2), also called CHK2 or RAD53, is a cell cycle checkpoint regulator and putative tumor suppressor which contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. CHEK2 is mapped on 22q12.1. CHEK2 was modified by phosphorylation and activated in response to ionizing radiation, and was also modified in response to hydroxyurea treatment. In response to DNA damage, CHEK2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Following gamma irradiation, CHEK2 phosphorylated PML on ser117, causing dissociation of the 2 proteins. |
| References: | 1.Ahn, J.-Y., Li, X., Davis, H. L., Canman, C. E. Phosphorylation of threonine 68 promotes oligomerization and autophosphorylation of the Chk2 protein kinase via the forkhead-associated domain. J. Biol. Chem. 277: 19389-19395, 2002. 2.Bartkova, J., Horejsi, Z., Koed, K., Kramer, A., Tort, F., Zieger, K., Guldberg, P., Sehested, M., Nesland, J. M., Lukas, C., Orntoft, T., Lukas, J., Bartek, J. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434: 864-870, 2005. 3.Bell, D. W., Varley, J. M., Szydlo, T. E., Kang, D. H., Wahrer, D. C. R., Shannon, K. E., Lubratovich, M., Verselis, S. J., Isselbacher, K. J., Fraumeni, J. F., Birch, J. M., Li, F. P., Garber, J. E., Haber, D. A. Heterozygous germ line hCHK2 mutations in Li-Fraumeni syndrome. Science 286: 2528-2531, 1999. |
| Additional info: | A synthetic peptide corresponding to a sequence at the N-terminal of human CHEK2, identical to the related rat and mouse sequences. |
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