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Products Polyclonal Anti-CCR10
| Catalogue number: | PA1491 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | CCR10 |
| Swiss prot: | P46092 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+ (cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27. |
| References: | 1. Homey, B., Alenius, H., Muller, A., Soto, H., Bowman, E. P., Yuan, W., McEvoy, L., Lauerma, A. I., Assmann, T., Bunemann, E., Lehto, M., Wolff, H., Yen, D., Marxhausen, H., To, W., Sedgwick, J., Ruzicka, T., Lehmann, P., Zlotnik, A.CCL27-CCR10 interactions regulate T cell-mediated skin inflammation.Nature Med. 8: 157-165, 2002. 2. Jarmin, D. I., Rits, M., Bota, D., Gerard, N. P., Graham, G. J., Clark-Lewis, I., Gerard, C.Cutting edge: identification of the orphan receptor G-protein-coupled receptor 2 as CCR10, a specific receptor for the chemokine ESkine.J. Immun. 164: 3460-3464, 2000. 3. Marchese, A., Docherty, J. M., Nguyen, T., Heiber, M., Cheng, R., Heng, H. H. Q., Tsui, L.-C., Shi, X., George, S. R., O'Dowd, B. F.Cloning of human genes encoding novel G protein-coupled receptors.Genomics 23: 609-618, 1994. |
| Additional info: | A synthetic peptide corresponding to a sequence at the C-terminal of human CCR10, different from the related rat and mouse sequences by one amino acid. |
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