Home  >  Products  >  Polyclonal Anti-CBS
Polyclonal Anti-CBS

Polyclonal Anti-CBS

Cat no: PA1542

Supplier: Boster Immunoleader
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Rabbit IgG polyclonal antibody for Cystathionine beta-synthase (CBS) detection. Tested with WB, IHC-P in Human.
Catalogue number: PA1542
Price: $200.00
Reactivities: Human
Applications: Immunohistochemistry, Western Blot
Size: 100ug/vial
Gene: CBS
Swiss prot: P35520
Form: Lyophilized
Format: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Storage temp: "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. "
Scientific background: CBS, Cystathionine Beta-Synthase , catalyzes the first irreversible step of transsulfuration.The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity. The CBS gene, which is mapped to chromosome 21q22 , contains 23 exons, ranging in size from 42 to 299 bp.The human CBS protein can substitute for the endogenous yeast CBS protein in Saccharomyces cerevisiae.The catalytic domain of the CBS protein is located in the N-terminal 409 amino acids, and a regulatory domain is located in the C-terminal 142 amino acids.A mutation that deletes the C-terminal 145 amino acids of CBS could restore activity of several CBS mutant alleles found in homocystinurics.
References: 1.Munke, M., Kraus, J., Watkins, P., Tanzi, R., Gusella, J., Millington Ward, A., Watson, M., Francke, U.Homocystinuria gene on human chromosome 21 mapped with cloned cystathionine beta-synthase probe and in situ hybridization of other chromosome 21 probes. (Abstract)Cytogenet. Cell Genet. 40: 706-707, 1985. 2.Kraus, J. P., Oliveriusova, J., Sokolova, J., Kraus, E., Vlcek, C., de Franchis, R., Maclean, K. N., Bao, L., Bukovska, G., Patterson, D., Paces, V., Ansorge, W., Kozich, V.The human cystathionine beta-synthase (CBS) gene: complete sequence, alternative splicing, and polymorphisms.Genomics 52: 312-324, 1998. 3.Kruger, W. D., Cox, D. R.A yeast system for expression of human cystathionine beta-synthase: structural and functional conservation of the human and yeast genes.Proc. Nat. Acad. Sci. 91: 6614-6618, 1994.
Additional info: A synthetic peptide corresponding to a sequence in the middle of human CBS.
See Full Product Listings >