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Products Polyclonal Anti-CA2
| Catalogue number: | PA1438 |
| Price: | $200.00 |
| Reactivities: | Human, Mouse, Rat |
| Applications: | Western Blot |
| Size: | 100ug/vial |
| Gene: | CA2 |
| Swiss prot: | P00918 |
| Form: | Lyophilized |
| Format: | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Storage temp: | "At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing. " |
| Scientific background: | Carbonic anhydrase II (CA2), is one of fourteen forms of human alpha Carbonic anhydrases. Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. Using Southern blot analysis and restriction endonucleases, it is determined that the CA2 gene consists of 7 exons. Using a mouse CA II cDNA hybridization probe with a panel of mouse-human cell hybrids, The CA2 gene was assigned to human chromosome 8. CA2 is expressed at high levels in osteoclasts during bone resorption. It is demonstrated in 2 in vitro culture systems that antisense RNA and DNA blocked expression of CA2 and led to decreased bone resorption. In the choroid plexus, CA2 supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid. |
| References: | 1. Laitala, T., Vaananen, H. K.Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H(+)-ATPase.J. Clin. Invest. 93: 2311-2318, 1994. 2. Venta, P. J., Shows, T. B., Curtis, P. J., Tashian, R. E.Polymorphic gene for human carbonic anhydrase II: a molecular disease marker located on chromosome 8.Proc. Nat. Acad. Sci. 80: 4437-4440, 1983. 3. Venta, P. J., Welty, R. J., Johnson, T. M., Sly, W. S., Tashian, R. E.Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107his-to-tyr): complete structure of the normal human CA II gene.Am. J. Hum. Genet. 49: 1082-1090, 1991. |
| Additional info: | A synthetic peptide corresponding to a sequence in the middle region of human CA2 , identical to the related mouse and rat sequence. |
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