Polyclonal Anti-ALOX12

Price

200.00 USD

Size

100ug/vial

Reactivities

Hum

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Funk, C. D., Furci, L., FitzGerald, G. A.Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase.Proc. Nat. Acad. Sci. 87: 5638-5642, 1990.\n2. Natarajan, R., Esworthy, R., Bai, W., Gu, J.-L., Wilczynski, S., Nadler, J.Increased 12-lipoxygenase expression in breast cancer tissues and cells. Regulation by epidermal growth factor.J. Clin. Endocr. Metab. 82: 1790-1798, 1997.\n3. Yoshimoto, T., Arakawa, T., Hada, T., Yamamoto, S., Takahashi, E.Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene.J. Biol. Chem. 267: 24805-24809, 1992.\n

Storage Temp

\"At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.\nAvoid repeated freezing and thawing. \n\"\n

Scientific Background

ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12(S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.