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T7 RNA Polymerase

T7 RNA Polymerase


T7 RNA Polymerase
catalyses the synthesis of RNA in the presence of double-stranded DNA containing a T7 promoter sequence.

It is isolated from an E. coli transformed by a plasmid containing the T7 RNA Polymerase gene.

This enzyme is provided in two concentrations in glycerol-based storage buffer containing: 20 mM potassium phosphate pH 7.5, 100 mM NaCl, 1.0 mM EDTA, 10 mM DTT, 0.2% Triton X-100 and 50% glycerol.

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