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Ribonuclease H


Supplier: Life Sciences Advanced Technologies
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Ribonuclease H
from E. coli.

Ribonuclease H is an endonuclease which specifically degrades the RNA moiety of a DNA:RNA hybrid, without affecting the unhybridized RNA. It does not affect double-stranded RNA or DNA.

It is provided in a 50% glycerol storage buffer.

Applications: Enabling the synthesis of second-strand cDNA by removal of the RNA; Used in conjunction with AMV RT and T7 RNA Polymerase in NASBA amplification of RNA.