Cycloheximide is an antibiotic, which is primary used for the isolation of dermatophytes from clinical materials. It induces death of eukaryote cells in an apoptosis-independent pathway. Cycloheximide acts as an elongation inhibitor during protein synthesis in eukaryotes but not prokaryotes by competition with the binding of ATP to the 60S ribosomal subunit. Mutations in the yeast gene CYH2 that encode to L29, a component of the 60S ribosomal subunit, can lead to Cycloheximide resistance. Cell Signaling Activators and Inhibitors; Protein Synthesis and Transport Inhibitors.
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