The family of SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Chemiluminescent HRP Substrate Reagent is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.