Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit

By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction.\nTrypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.\nImmunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.

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SPECIFICATIONS

Catalog Number

KTE62208

Size

48T, 96T, 96T*5, 96T*50

Applications

ELISA

Reactivities

Hum

Gene Id

151056

Accession

Q6P1J6

Additional Info

Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLB1. No significant cross-reactivity or interference between Human PLB1 and analogues was observed.