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HT1080 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
SH-SY5Y cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
COLO205 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
THP-1 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
MCF7 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
721_B cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
Raji cells was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
Hela cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
293T cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
Daudi cells was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
K562 cells was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
Jurkat cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
HepG2 cell was lysed in RIPA buffer and mixed with equivalent volumes of 2X SDS-PAGE loading buffer prior to lyophilization.
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A solution in 10 mM Tris-Cl and 1 mM EDTA, pH 8.0.
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