1. WST-1 Cell Proliferation and Cytotoxicity Assay Kit is a sensitive and accurate assay for cell cytotoxicity and proliferation. The kit components are sufficient for performing up to 100 assays.
2. WST-1 assay is much like MTT assay and the MTS assay, they are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple yellow. A main application allows assessing the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth.
3. This kit is very convenience to use. It is unnecessary to use isotope, to wash and collect cells, as well as to solute formazan. The entire step can be done in only one 96-well plate.
4. No interrupt with Phenol red and serum.
5. WST-1 is not toxic to cells. After adds WST-1, it can be read by coated wells repeatedly in different time. In this way, the detect time will be more flexible to find the best detect time.
6. The kit components are sufficient for performing up to 100 assays.